Journal: Pflugers Archiv : European journal of physiology

Article Title: Temporal and spatial summation of laser heat stimuli in cultured nociceptive neurons of the rat.

doi: 10.1007/s00424-022-02728-1

Figure Lengend Snippet: Fig. 1 Experimental setup for investigation of diode laser-induced calcium transients in cultured nociceptive neurons of rats. Laser pulses were applied from above through the cover glasses directly to the neurons that adhere to the back of the cover glasses. Extra- cellular solution was perfused below the neurons through the small space between the cover glass and the bottom of the culture dish. A FURA-2 loaded cells as visualized in the usual XY-plane normally used in calcium imaging experiments (left) as well as after three- dimensional XYZ-reconstruction illustrating the cells hanging upside down from the cover glass (right). Note one mid-size DRG neuron surrounded by several smaller satellite cells. B Sketch of the experi- mental setup; dotted lines indicate magnified inset demonstrating the laser stimulation of hanging cells from above; red line indicates weaker refraction of the invisible NIR laser as compared to the vis- ible pilot laser (grey line). C Photography of the experimental set-up. 1 collimator with glass fiber, 2 micromanipulator, 3 culture dish with cover glass, 4 inlet, 5 outlet, 6 extraction system to keep the surface of the cover glass dry

Article Snippet: The fiber collimator that focused the two laser radiations was moved by a computer-controlled motorized micromanipulator (Sutter MP-285, Sutter Instruments, Novato, CA, USA) in 40-nm steps.

Techniques: Cell Culture, Imaging, Extraction